Diversity of Streptomyces in mangrove ecosystem of Bhitarkanika
Collection of soil samples. Treatment of soil samples and selective isolation of rare actinomycetes. Characterization of non-streptomycete actinomycetes. Screening for bioactivity. Anti-Quorum sensing activity. Table 1 PCR primers used in this study. Open in a separate window. DNA extraction, sequencing and analysis.
Antibacterial activity of soil actinomycetes from the mangrove Avicennia marina
Physico-chemical screening. Anticancer activity. Table 2 Recovery of rare actinomycetes colonies using selective isolation methods. Treatment Media Antibiotics used No. Identification of rare actinomycetes. Table 3 Grouping of isolates into genera on the basis of morphological and chemotaxonomic properties. Genus No. Table 4 Similarity of the rare actinobacterial isolates to the closest cultivated species and their Genbank accession numbers. Isolate Genbank accession No.
Secondary screening. Physicochemical screening. Table 5 Summary of culturable rare actinomycete isolates from Indian forest soils. Selective isolation of rare actinomycetes. Molecular evolution of aromatic polyketides and comparative sequence analysis of polyketide ketosynthase and 16S ribosomal DNA genes from various Streptomyces species. Appl Environ Microbiol ; 68 — Isolation, identification and molecular characterization of rare actinomycetes from mangrove ecosystem of Nizampatnam.
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The hydrolysate obtained was transferred to centrifuge tube after cooling. The pH was adjusted to 5 after which the centrifugation was performed. The immense biological diversity in soil has led to the recognition of large number of chemical entities in soil. The soil sample for the work was collected from Western Ghats as they are known to harbour a very rich community of flora and fauna. Hence the soil present there is considered to be rich in various types of microbial flora maintaining the ecological diversity.
Due to the rainy season at the time of sample collection the amount and the type of microbial diversity present in soil might vary from that found during summer and winter season. The isolated Actinomycetes were found dominant over fungi and bacteria. This variation in www. Hence it can be considered that the Actinomycetes here are might be secreting some secondary metabolites which are inhibitory to bacteria.